Friday, March 5, 2010
CCR Biotech Workshop #2
What a day! We learned so much. Moreover, we were so inspired. Today we learned to isolate plasmid DNA(pcDNA-luc) then digest them using restriction enzymes: HindIII and NgoMIV. Our gel did not really show to many bands (maybe we did not isolate enough plasmids) so below is Cindy's. BTW, Cindy thanks for staying with us last Friday.
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Prompt: What is the most interesting lecture/concept/activity you have seen so far in the last two workshop meetings that you wanted to share with your students? (This could be a lab activity, lecture, or even "meet a scientist")
I really enjoyed the plasmid prep, purification, and digest. I would like to do it about 10 more times so I'm really confident about the procedure and feel comfortable replicating it with students! This is the 3rd time I've done it within an 18 month period - so lots of gaps in the application. I would like to do a restrictive enzyme digest with my students. I just wonder about how do you do it with 35 students, and are they mature enough to handle such a detailed lab? I don't really think so. I'm thinking of breaking the lab into simpler steps, perhaps starting with a micropipette color wheel lab and seeing how that goes.
Dr. Shohet's talk on hypoxia inducement was very interesting. I found him more understandable than Dr. Stokes. I shared with my students the petri dish slide with the mouse heart cells that were beating autonomously - that was amazing! I'd like to see them up close in real life. I can see talking about the production of red blood cells and how the gene that produces them is usually turned off, but if you live at a high altitude this gene is turned on to help create carry more oxygen throughout your body. Perhaps this example could be brought up in the biology unit on adaptations...It was a very interesting day.
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