This is a log of my professional activities while working at Baldwin High School as a science teacher. - Amy Ancheta
Tuesday, March 9, 2010
CCR Workshop Reflection
What is the most interesting lecture/concept/activity you have seen so far in the last two workshop meetings that you wanted to share with your students? (This could be a lab activity, lecture, or even "meet a scientist").
I was so excited to learn how to isolate plasmid DNA. We currently perform tranformation with pGLO. If we can isolate out pGLO after the transformation, we, no longer, need to buy any more plasmids. Just need to isolate and freeze. Hooray!
I will perform plasmid isolation (on our pGLO E.coli) with my students when they return from the spring break. So exciting to learn something that is so applicable in my class.
Hmmm... hard to select just ONE thing I thought was most interesting. I loved being able to do the lab procedure we did last week - the hands-on work and getting to know my lab partners - both very enjoyable. Thus far I have been able to share with my students the topics covered in the "meet a scientist" sessions. Brian and I have been trying to figure out how we can take some of our students over to Amy's lab to do some of the lab procedures.
Nancy Halley aka "anon" said... Prompt: What is the most interesting lecture/concept/activity you have seen so far in the last two workshop meetings that you wanted to share with your students? (This could be a lab activity, lecture, or even "meet a scientist") I really enjoyed the plasmid prep, purification, and digest. I would like to do it about 10 more times so I'm really confident about the procedure and feel comfortable replicating it with students! This is the 3rd time I've done it within an 18 month period - so lots of gaps in the application. I would like to do a restrictive enzyme digest with my students. I just wonder about how do you do it with 35 students, and are they mature enough to handle such a detailed lab? I don't really think so. I'm thinking of breaking the lab into simpler steps, perhaps starting with a micropipette color wheel lab and seeing how that goes.
Dr. Shohet's talk on hypoxia inducement was very interesting. I found him more understandable than Dr. Stokes. I shared with my students the petri dish slide with the mouse heart cells that were beating autonomously - that was amazing! I'd like to see them up close in real life. I can see talking about the production of red blood cells and how the gene that produces them is usually turned off, but if you live at a high altitude this gene is turned on to help create carry more oxygen throughout your body. Perhaps this example could be brought up in the biology unit on adaptations...It was a very interesting day.
Very interesting Day 2 at JABSOM. Of all the activities and lectures that we have been exposed to, I think what is most applicable to Cecelia and my students is that we can explain to them that there are ways to "cut" DNA into pieces and the pieces of DNA can be more easily studied. As Cecelia stated, it would be a great experience for our students to try some of the lab techniques in Amy's room. I found the plasmid isolation/digestion lab fun, mainly because it was "hands on"(even though we didn't get the results we were looking for).
For me personally, I have really enjoyed listening to the scientists at CCR present information on their current research and I am looking forward to more presentations.
4 comments:
I was so excited to learn how to isolate plasmid DNA. We currently perform tranformation with pGLO. If we can isolate out pGLO after the transformation, we, no longer, need to buy any more plasmids. Just need to isolate and freeze. Hooray!
I will perform plasmid isolation (on our pGLO E.coli) with my students when they return from the spring break. So exciting to learn something that is so applicable in my class.
Hmmm... hard to select just ONE thing I thought was most interesting. I loved being able to do the lab procedure we did last week - the hands-on work and getting to know my lab partners - both very enjoyable. Thus far I have been able to share with my students the topics covered in the "meet a scientist" sessions. Brian and I have been trying to figure out how we can take some of our students over to Amy's lab to do some of the lab procedures.
Nancy Halley aka "anon" said...
Prompt: What is the most interesting lecture/concept/activity you have seen so far in the last two workshop meetings that you wanted to share with your students? (This could be a lab activity, lecture, or even "meet a scientist")
I really enjoyed the plasmid prep, purification, and digest. I would like to do it about 10 more times so I'm really confident about the procedure and feel comfortable replicating it with students! This is the 3rd time I've done it within an 18 month period - so lots of gaps in the application. I would like to do a restrictive enzyme digest with my students. I just wonder about how do you do it with 35 students, and are they mature enough to handle such a detailed lab? I don't really think so. I'm thinking of breaking the lab into simpler steps, perhaps starting with a micropipette color wheel lab and seeing how that goes.
Dr. Shohet's talk on hypoxia inducement was very interesting. I found him more understandable than Dr. Stokes. I shared with my students the petri dish slide with the mouse heart cells that were beating autonomously - that was amazing! I'd like to see them up close in real life. I can see talking about the production of red blood cells and how the gene that produces them is usually turned off, but if you live at a high altitude this gene is turned on to help create carry more oxygen throughout your body. Perhaps this example could be brought up in the biology unit on adaptations...It was a very interesting day.
Very interesting Day 2 at JABSOM. Of all the activities and lectures that we have been exposed to, I think what is most applicable to Cecelia and my students is that we can explain to them that there are ways to "cut" DNA into pieces and the pieces of DNA can be more easily studied. As Cecelia stated, it would be a great experience for our students to try some of the lab techniques in Amy's room. I found the plasmid isolation/digestion lab fun, mainly because it was "hands on"(even though we didn't get the results we were looking for).
For me personally, I have really enjoyed listening to the scientists at CCR present information on their current research and I am looking forward to more presentations.
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